乐虎游戏

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  2020NIAN8YUE10RI23DIAN,《Nature Biotechnology》ZAZHIZAIXIANFABIAOLETIWEI《GONGJUJIAOGUANGCHANGXIANWEIJINGDUIXIAOSHUHEBANMAYUDANAOKUAISUTICHENGXIANG》DEYANJIULUNWEN,GAIYANJIUYOUZHONGGUOKEXUEYUANNAOKEXUEYUZHINENGJISHUZHUOYUECHUANGXINZHONGXIN(SHENJINGKEXUEYANJIUSUO)、SHANGHAINAOKEXUEYULEINAOYANJIUZHONGXIN、SHENJINGKEXUEGUOJIAZHONGDIANSHIYANSHIWANGKAIYANJIUZUWANCHENG。GAIYANJIUFAZHANLEYIZHONGXINXINGTICHENGXIANGJISHU: GONGJUJIAOGUANGCHANGXIANWEIJING(Confocal light field microscopy),KEYIDUIHUOTIDONGWUSHENBUNAOZUZHIZHONGSHENJINGHEXUEGUANWANGLUOJINXINGKUAISUDAFANWEITICHENGXIANG。 

乐虎游戏  KUANAOQUDAGUIMODESHENJINGYUANRUHEZHENGHEXINXIBINGYINGXIANGXINGWEISHISHENJINGKEXUEZHONGDEHEXINWENTI,JIEDAZHEIGEWENTIXUYAOZAIGENGGAOSHIKONGFENBIANLVSHANGBUZHUODALIANGSHENJINGYUANHUODONGDONGTAIBIANHUADEGONGJU。GONGJUJIAOXIANWEIJINGHESHUANGGUANGZIXIANWEIJINGDENGYUNYONGYUHUOTINAOCHENGXIANGDECHUANTONGGONGJUJIYUDIANSAOMIAO,SHIJIANFENBIANLVJIAODI,NANYIYANJIUDAFANWEINAOQUZHONGSHENJINGYUANDEKUAISUBIANHUA。YINCI,JINNIANLAIRENMENYIZHIZHILIYUKAIFAGENGKUAIDECHENGXIANGFANGFA。ZAIDUOZHONGXINJISHUZHONG,GUANGCHANGXIANWEIJINGYOUQIJUYOUQIANLI,DEDAOLEGUANGFANGUANZHU。QITEDIANZAIYUKEYIZAIXIANGJIDEDANCIPUGUANGSHUNJIAN,JILULAIZIWUTIBUTONGSHENDUDEXINHAO,TONGGUOFANJUANJISUANFAZHONGGOUCHUZHENGGESANWEITI,SHIXIANKUAISUTICHENGXIANG,ZAIXIANCHONG、BANMAYUYOUYUDENGXIAOXINGMOSHIDONGWUSHANGYIHUODECHUBUYINGYONG。 

  CHUANTONGGUANGCHANGXIANWEIJINGCUNZAILIANGGENANYIJIEJUEDEWENTI,XIANZHILEQIZAISHENGWUCHENGXIANGSHANGDEGUANGFANYINGYONG。SHOUXIAN,ZHONGGOUDEJIEGUOHUICHUXIANSHIZHEN。2017NIANWANGKAIYANJIUZUYANFADEXINXINGKUOZENGSHICHANGGUANGCHANGXIANWEIJING(eXtended field-of-view Light Field Microscopy, XLFM)YOUXIAOJIEJUELEZHEIGEWENTI,BINGCHENGGONGYINGYONGYUZIYOUXINGWEIBANMAYUYOUYUDEQUANNAOSHENJINGYUANGONGNENGCHENGXIANGSHANG,SHOUCISANWEIJILULEBANMAYUYOUYUZAIWANZHENGBUSHIXINGWEIZHONGDEQUANNAOSHENJINGYUANHUODONGDEBIANHUA。QICI,XIANYOUGUANGCHANGXIANWEICHENGXIANGJISHUQUEFAGUANGXUEQIEPIANNENGLI,WUFADUIJIAOHOUZUZHI,BIRUXIAOSHUDEDANAOJINXINGCHENGXIANG。RANGGUANGCHANGXIANWEIJINGJUYOUGONGJUJIAOXIANWEIJINGYIYANGDEGUANGXUEQIEPIANNENGLI,LVCHUDAYANGPINZHONGJIAOCENGZHIWAIDEBEIJINGXINHAOLAITIGAOXINZAOBI,SHITIGAOCHENGXIANGZHILIANG、KEGUANGFANYINGYONGDEGUANJIANSUOZAI。 

  RANER,CHUANTONGGONGJUJIAOXIANWEIJINGCAIYONGJIGUANGZHUDIANSAOMIAOHEGONGEDIANZHENKONGJIANCELAIJIANGDIJIAOMIANWAIZAOSHENGDECELVEBUSHIYONGYUSANWEIGUANGCHANGXIANWEIJING。MIANDUIZHEIYITIAOZHAN,YANJIUTUANDUICHUANGXINTICHUGUANGYIGONGJUJIAOJIANCEDEGAINIAN,SHIQIKEYIYUGUANGCHANGXIANWEIJINGDESANWEICHENGXIANGCELVEJIEHE,ZAIBUXISHENGTICHENGXIANGSUDUDEQIANTIXIAYOUXIAOLVCHUBEIJINGZAOSHENG,DAFUDUTIGAOLELINGMINDUHEFENBIANLV。ZHEIZHONGXINXINGDEGUANGCHANGXIANWEICHENGXIANGJISHUCHENGWEIGONGJUJIAOGUANGCHANGXIANWEIJING。    

  图1(上)共聚焦光场显微镜原理示意图。(下)不同于传统光场显微镜,共聚焦光场显微镜采用片状照明,选择性激发样本的一部分,在垂直照明的方向上扫描,采集到的信号被遮挡板过滤掉焦层范围之外的部分。对采集到的图像进行重构可以得到焦层内的三维信息。 

  研究团队在不同动物样品上测试了共聚焦光场显微镜的成像能力。首先,团队成员对包埋的活体斑马鱼幼鱼进行全脑钙成像,对比共聚焦和传统光场显微镜的成像结果,发现加入光学切片能力后,图像分辨率和信号噪声比显著提高,可以检测到更多较弱的钙活动。进一步的,将共聚焦光场显微镜和高速三维追踪系统结合,对自由行为的斑马鱼幼鱼进行全脑钙成像,在? 800 μm x 200 μm的体积内达到了2 x 2 x 2.5 μm3的空间分辨率和6Hz的时间分辨率。受益于更高的分辨率和灵敏度,可以识别出斑马鱼幼鱼在捕食草履虫过程中单个神经元的钙离子活动的变化。 

   

  图 2 (左)斑马鱼幼鱼捕食行为的一个例子。0s 为斑马鱼吞食草履虫的时刻。(右)左图斑马鱼捕食行为中,共聚焦光场显微镜记录到的两个不同脑区的神经元活动。箭头所指为过程中激活的单个神经元。 

  JINER,TUANDUICHENGYUANYANZHENGLEGONGJUJIAOGUANGCHANGXIANWEIJINGDUIXIAOSHUDANAODECHENGXIANGXIAOGUO,DUIQINGXINGXIAOSHUDESHIPICENGJINXINGGAICHENGXIANG,KEYITONGSHIJILU? 800 μm x 150 μmDETIJINEIJINQIANGESHENJINGYUANDEHUODONG,ZUISHENKEDAYUE400 μm,BINGQIELIANXU5XIAOSHIYISHANGWENDINGJILUCHAOGUO10WANZHEN,MEIYOUMINGXIANDEGUANGPIAOBAI。TUANDUICHENGYUANJINYIBUCHANGSHISHIYONGGONGJUJIAOGUANGCHANGXIANWEIJINGDUISHUNAOZHONGDEXUEXIBAOJINXINGCHENGXIANG,SHENDUKEDA600 μm,PAISHESUDU70 Hz,TONGSHIJILUSHANGQIANGENXUEGUANFENZHIZHONGQUNTIXUEXIBAODELIUDONGQINGKUANGBINGJISUANXUEXIBAODESUDU,XIANGBIZHIQIANDECHUANTONGCHENGXIANGFANGFATONGLIANGTIGAOLEBAIYUBEI。    

  图 3 (左)共聚焦光场显微镜拍摄得到的小鼠视皮层中的复杂血管网络。6个在不同深度拍摄的体积连接为一个深度达600 μm的三维结构。(中)100 μm到250 μm深度血管网络的平面投影,颜色代表不同血管分支中血细胞的平均流速。(右)图中箭头所指的区域中五个血管分支在一段时间内流过血细胞数量的计数。 

  YANJIUTUANDUIZAIZIYOUXINGWEIDEBANMAYUYOUYUHEXIAOSHUDANAOSHANGZHENGMINGLEGONGJUJIAOGUANGCHANGXIANWEIJINGYOUGENGGAODEFENBIANLVHELINGMINDU,ZHEIWEIYANJIUDAFANWEISHENJINGWANGLUOHEXUEGUANWANGLUODEGONGNENGTIGONGLEXINDEGONGJU。TONGSHI,GAIJISHUBUJINSHIYONGNAOZUZHIDECHENGXIANG,HAIKEYIGENJUSUOXUCHENGXIANGDEYANGPINZHONGLEILINGHUODIAOZHENGFENBIANLV、CHENGXIANGFANWEIHESUDU,YINGYONGZAIQITAHOUZUZHIDEKUAISUDONGTAICHENGXIANGZHONG。 

乐虎游戏  GAIYANJIUZAIWANGKAIYANJIUYUANDEZHIDAOXIA,ZHUYAOYOUBOSHIYANJIUSHENGZHANGZHENKUN、BAILUHEZHULIYANJIUYUANCONGLINGONGTONGWANCHENG,WANGKAIYANJIUZUYUPENG、ZHANGTIANLEI、ZHONGKEDABENKESHENGSHIWANZHUO、DUJIULINYANJIUZULIFUNINGYEZUOCHULEZHONGYAOGONGXIAN,DUJIULINYANJIUYUANCANYUHEZUOBINGJIYUZHIDAOYIJIAN。GAIYANJIUDEDAOLEZHONGKEYUANNAOZHIZHUOYUEZHONGXINSHIYANDONGWUPINGTAIDEDALIZHICHI。YANJIUHUODELELAIZIZHONGGUOKEJIBU、ZHONGGUOKEXUEYUAN、GUOJIAZIRANKEXUEJIJINWEIYUANHUIHESHANGHAISHIDEXIANGMUZIZHU。 

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